![]() Powered by django-wiki, an open source application under the GPLv3 license. Typical diluions are 10-fold dilutions each dilution is 1/10th. The process of transferring aliquot is repeated until the desired dilution concentration is achieved.Īlthough serial dilution requires more preparation, it will give more accurate dilutions, provided that your technique is precise. An undiluted (stock) solution is termed the 100 dilution. Then 1 mL (aliquot) of the second tube is taken and transferred to the third tube. 1 mL of stock is transferred to the second tube that contains 9 mL of diluent (e.g water). For example, you may need to dilute a solution by a factor of one million. Similarly, for a 15X dilution, it will be 1 part of the solution, 14 parts of the diluent.įigure 1: Serial dilution. This technique is used when a high degree of dilution is required. When it is a 10X dilution, it means that it is 1 part of the solution, 9 parts of the diluent (usually it is water). This means we can set up 3 tubes to perform the serial dilution. We can split up 1000X into multiple factors: 10 X 10 X 10 So if we need 10 ml of a 1 mM solution, our dilution factor is:ĭilution factor= 1/ (final concentration/ initial concentration) Whereas if it was done by serial dilution, we first look at the final volume and concentrations needed, then determine the dilution factor. It is a basic protocol for nearly every life science lab, and is used routinely to. In these cases, direct dilution is not very helpful because it would be difficult to pipette exactly 9.99 ml. A serial dilution is the sequential dilution of a substance in solution. Serial dilutions are useful when we want to obtain a very diluted solution from very concentrated stock solutions.įor example, if we want to obtain 10 ml of 1 mM solution from a 1M stock solution By direct dilution: The traditional serial dilution method involves using capped glass test tubes filled with 9 ml of sterile diluent, where 1 ml of the sample is pipetted into the.
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